RNA-FISH for investigating Cultural Heritage Microbiology- Autofluorescence of the materials and fluorophores selection.

This work was focused on: i) the investigation of the autofluorescence of various materials that are found forming part of Cultural Heritage objects (parchment, paper, wood, fabric, rock, mortar and plastic among others) by epifluorescence microscopy using fluorescence flter sets targeted at commonly u...

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Detalhes bibliográficos
Autor principal: González-Pérez, Marina (author)
Outros Autores: Vieira, Ricardo (author), Branco, Patricia (author), Candeias, António (author), Caldeira, Ana Teresa (author)
Formato: article
Idioma:por
Publicado em: 2018
Assuntos:
Texto completo:http://hdl.handle.net/10174/21742
País:Portugal
Oai:oai:dspace.uevora.pt:10174/21742
Descrição
Resumo:This work was focused on: i) the investigation of the autofluorescence of various materials that are found forming part of Cultural Heritage objects (parchment, paper, wood, fabric, rock, mortar and plastic among others) by epifluorescence microscopy using fluorescence flter sets targeted at commonly used fluorophores (TRITC/CY5/6-FAM); ii) the detection of RNA-FISH stained microbial cells on the selected materials or in the presence of residual particles.