Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae

Abstract We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting...

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Bibliographic Details
Main Author: Pereira, Hugo (author)
Other Authors: Barreira, Luísa (author), Mozes, Andre (author), Florindo, C. (author), Polo, Cristina (author), Duarte, Catarina V. (author), Custódio, Luísa (author), Varela, J. (author)
Format: article
Language:eng
eng
Published: 2012
Online Access:http://hdl.handle.net/10400.1/917
Country:Portugal
Oai:oai:sapientia.ualg.pt:10400.1/917
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Summary:Abstract We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.

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