Interlaboratory coverage test on plant food bioactive compounds and their metabolites by mass spectrometry-based untargeted metabolomics

Bioactive compounds present in plant-based foods, and their metabolites derived from gut microbiota and endogenous metabolism, represent thousands of chemical structures of potential interest for human nutrition and health. State-of-the-art analytical methodologies, including untargeted metabolomics...

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Bibliographic Details
Main Author: Koistinen, Ville Mikael (author)
Other Authors: da Silva, Andreia Bento (author), Abrankó, László (author), Low, Dorrain (author), Villalba, Rocio Garcia (author), Barberán, Francisco Tomás (author), Landberg, Rikard (author), Savolainen, Otto (author), Alvarez-Acero, Inmaculada (author), de Pascual-Teresa, Sonia (author), Van Poucke, Christof (author), Almeida, Conceição (author), Petrásková, Lucie (author), Valentová, Kateřina (author), Durand, Stephanie (author), Wiczkowski, Wiesław (author), Szawara-Nowak, Dorota (author), González-Domínguez, Raúl (author), Llorach, Rafael (author), Andrés-Lacueva, Cristina (author), Aura, Anna Marja (author), Seppänen-Laakso, Tuulikki (author), Hanhineva, Kati (author), Manach, Claudine (author), Bronze, Maria Rosário (author)
Format: article
Language:eng
Published: 2019
Subjects:
Mass spectrometry
Method development
Phytochemicals
Endocrinology, Diabetes and Metabolism
Biochemistry
Molecular Biology
SDG 3 - Good Health and Well-being
Online Access:https://doi.org/10.3390/metabo8030046
Country:Portugal
Oai:oai:run.unl.pt:10362/68111
Description
Summary:Bioactive compounds present in plant-based foods, and their metabolites derived from gut microbiota and endogenous metabolism, represent thousands of chemical structures of potential interest for human nutrition and health. State-of-the-art analytical methodologies, including untargeted metabolomics based on high-resolution mass spectrometry, are required for the profiling of these compounds in complex matrices, including plant food materials and biofluids. The aim of this project was to compare the analytical coverage of untargeted metabolomics methods independently developed and employed in various European platforms. In total, 56 chemical standards representing the most common classes of bioactive compounds spread over a wide chemical space were selected and analyzed by the participating platforms (n = 13) using their preferred untargeted method. The results were used to define analytical criteria for a successful analysis of plant food bioactives. Furthermore, they will serve as a basis for an optimized consensus method.

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