| Summary: | Actinidia arguta is a plant that produces a fruit commonly known as Kiwiberry. This fruit exhibits a remarkable chemical composition and beneficial health effects. However, during its production diferente by-products, such as leaves, are generated. This by-product has revealed to be na excelente source of active ingredientes, having the potential to be incorporated in new pharmaceutical, nutraceutical or cosmetic products. The main objective of this work was to evaluate the effects of temperature (five between 1100c and 1600c) on the bioactivity, ability to scavenge free radicals and phenolic profile of extracts obtained from A. arguta leaves by subcritical water extraction (SWE), a sustainable and environmentally friendly technique. Additionally, the antibacterial capacity as well as the antiproliferative activity on intestinal cell lines were screened. Finally, a cosmetic formulation with the most promising extract was developed, using the Response Surface Methodology (RSM) mathematical model. All extracts displayed antioxidante and antiradical activities against free radicals. Interesting results were also observed regarding the total phenolic (TPC) and flavonoid contentes (TFC). The condition of 1230C achieved the best results in almost assays. However, no antibacterial activity was observed. Regarding the cell viability assays, the extracts did not lead to a decrease of HT29-MTX cells viability, while in Caco-2 cells a slightly decrease in the presence of the extract obtained at 1600C was observed. Regarding the phenolic profile, all the extracts revealed the presence of phenolic acids and flavonoids, mostly gallic, chlorogenic, protocatechuic and caffeic acids. Finally, the mathematical model was applied to determine the optimal amounts of excipientes (carbopol 940:0.3286 g; glycerin: 15 g; cetiol V: 13.80 g) for a cosmetic formulation produced with the best extract, aiming to predict these variables influence on the formulation behavior results. Satisfactory results were achieed, allowing to conclude the possibility of use this extract as a source of active ingredientes in cosmetic formulations.
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