| Resumo: | EFG1 is a central transcriptional regulator of filamentation that is an important virulence factor of Candida albicans. This study serves to assess in vivo the applicability of the anti-EFG1 2-OMethylRNA oligomer for inhibiting Candida albicans filamentation and to attenuate candidiasis, using the Galleria mellonella model. For that, larvae infected with a lethal concentration of C. albicans cells were treated with a single-dose and with a double-dose of the anti-EFG1 2OMe oligomer (at 40 and 100 nM). The anti-EFG1 2OMe oligomer toxicity and effect on larvae survival was evaluated. No evidence of anti-EFG1 2OMe oligomer toxicity was observed and the treatment with double-dose of 2OMe oligomer empowered the larvae survival over 24 h (by 90-100%) and prolonged its efficacy until 72 h of infection (by 30%). Undoubtedly, this work validates the in vivo therapeutic potential of anti-EFG1 2OMe oligomer for controlling C. albicans infections.
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