Development and application of a novel peptide nucleic acid probe for the specific detection of helicobacter pylori in gastric biopsy specimens

In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is hig...

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Bibliographic Details
Main Author: Guimarães, N. (author)
Other Authors: Azevedo, N. F. (author), Figueiredo, C. (author), Keevil, C. W. (author), Vieira, M. J. (author)
Format: article
Language:eng
Published: 2007
Subjects:
Science & Technology
Online Access:http://hdl.handle.net/1822/6999
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/6999
Description
Summary:In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffinembedded gastric biopsy specimens.

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