Species-specific real-time PCR cell number quantification of the bloom forming cyanobacterium Planktothrix agardhii

A species-specific method to detect and quantify Planktothrix agardhii was developed by combining the SYBR Green I real-time polymerase chain reaction technique with a simplified DNA extraction procedure for standard curve preparation. Newly designed PCR primers were used to amplify a specific fragm...

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Bibliographic Details
Main Author: Churro, Catarina (author)
Other Authors: Pereira, Paulo (author), Vasconcelos, Vitor (author), Valério, Elisabete (author)
Format: article
Language:eng
Published: 2012
Subjects:
Online Access:http://hdl.handle.net/10400.18/1119
Country:Portugal
Oai:oai:repositorio.insa.pt:10400.18/1119