Optimizing locked nucleic acid/2-O-methyl-RNA fluorescence in situ hybridization (LNA/2OMe-FISH) for bacterial detection

Despite the successful application of locked nucleic acid/2-O-methyl- RNA fluorescence in situ hybridization (LNA/2OMe-FISH) procedures for bacteria detection, there is a lack of knowledge on the properties that affect hybridization. Such information is crucial for the rational design of the protoco...

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Bibliographic Details
Main Author: Azevedo, Andreia S. (author)
Other Authors: Sousa, I. M. (author), Fernandes, R. (author), Azevedo, N. F. (author), Almeida, Carina (author)
Format: conferenceObject
Language:eng
Published: 2018
Online Access:http://hdl.handle.net/1822/56854
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/56854