Cloning, characterization, and tissue distribution of prolactin receptor in the sea bream (Sparus aurata)

The prolactin receptor (PRLR) was cloned and its tissue distribution characterized in adults of the protandrous hermaphrodite marine teleost, the sea bream (Sparus aurata). An homologous cDNA probe for sea bream PRLR (sbPRLR) was obtained by RT-PCR using gill mRNA. This probe was used to screen inte...

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Bibliographic Details
Main Author: Santos, Cecilia (author)
Other Authors: Ingleton, P. M. (author), Cavaco, J. E. B. (author), Kelly, P. A. (author), Edery, M. (author), Power, Deborah (author)
Format: article
Language:eng
Published: 2014
Subjects:
Prolactin receptor
Marine teleost
Osmoregulation
Reproduction
Online Access:http://hdl.handle.net/10400.1/5387
Country:Portugal
Oai:oai:sapientia.ualg.pt:10400.1/5387
Description
Summary:The prolactin receptor (PRLR) was cloned and its tissue distribution characterized in adults of the protandrous hermaphrodite marine teleost, the sea bream (Sparus aurata). An homologous cDNA probe for sea bream PRLR (sbPRLR) was obtained by RT-PCR using gill mRNA. This probe was used to screen intestine and kidney cDNA libraries from which two overlapping clones (1100 and 2425 bp, respectively) were obtained.

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