Highly dynamic host actin reorganization around developing Plasmodium inside hepatocytes

Plasmodium sporozoites are transmitted by Anopheles mosquitoes and infect hepatocytes, where a single sporozoite replicates into thousands of merozoites inside a parasitophorous vacuole. The nature of the Plasmodium-host cell interface, as well as the interactions occurring between these two organis...

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Bibliographic Details
Main Author: Gomes-Santos, Carina S S (author)
Other Authors: Itoe, Maurice A (author), Afonso, Cristina (author), Henriques, Ricardo (author), Gardner, Rui (author), Sepúlveda, Nuno (author), Simões, Pedro D (author), Raquel, Helena (author), Almeida, António Paulo (author), Moita, Luis F (author), Frischknecht, Friedrich (author), Mota, Maria M (author)
Format: article
Language:eng
Published: 2016
Subjects:
Actin Cytoskeleton
Actins
Animals
Cells, Cultured
Gelsolin
Green Fluorescent Proteins
Hepatocytes
Host-Parasite Interactions
Humans
Kinetics
Liver
Mice
Mice, Inbred BALB C
Organisms, Genetically Modified
Plasmodium
Protein Multimerization
Tubulin
Online Access:http://hdl.handle.net/10400.7/649
Country:Portugal
Oai:oai:arca.igc.gulbenkian.pt:10400.7/649
Description
Summary:Plasmodium sporozoites are transmitted by Anopheles mosquitoes and infect hepatocytes, where a single sporozoite replicates into thousands of merozoites inside a parasitophorous vacuole. The nature of the Plasmodium-host cell interface, as well as the interactions occurring between these two organisms, remains largely unknown. Here we show that highly dynamic hepatocyte actin reorganization events occur around developing Plasmodium berghei parasites inside human hepatoma cells. Actin reorganization is most prominent between 10 to 16 hours post infection and depends on the actin severing and capping protein, gelsolin. Live cell imaging studies also suggest that the hepatocyte cytoskeleton may contribute to parasite elimination during Plasmodium development in the liver.

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