Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria

Fluorescence in situ Hybridization (FISH) is a versatile, widespread and widely-used technique in microbiology. The first step of FISH fixation/permeabilization is crucial to the outcome of the method. This work aimed to systematically evaluate fixation/permeabilization protocols employing ethanol,...

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Detalhes bibliográficos
Autor principal: Rui Rocha (author)
Outros Autores: Carina Almeida (author), Nuno F. Azevedo (author)
Formato: article
Idioma:eng
Publicado em: 2018
Texto completo:https://hdl.handle.net/10216/115925
País:Portugal
Oai:oai:repositorio-aberto.up.pt:10216/115925