Optimization of peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria: the effect of pH, dextran sulfate and probe concentration

Fluorescence in situ hybridization (FISH) is a molecular technique widely used for the detection and characterization of microbial populations. FISH is affected by a wide variety of abiotic and biotic variables and the way they interact with each other. This is translated into a wide variability of...

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Bibliographic Details
Main Author: Rocha, Rui (author)
Other Authors: Santos, Rita S. (author), Pedro, Madureira (author), Almeida, Carina (author), Azevedo, Nuno F. (author)
Format: article
Language:eng
Published: 2016
Subjects:
PNA-FISH
Eubacteria
Dextran sulfate
pH
PNA EUB338
Engenharia e Tecnologia::Biotecnologia Industrial
Science & Technology
Online Access:http://hdl.handle.net/1822/41403
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/41403

Internet

http://hdl.handle.net/1822/41403

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