Synthetic biology to engineer bacteriophage genomes

Recent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized bacteriophages with new functionalities. Bacteriophages have been engineered towards a wide range of applications including pathogen control and detection, target...

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Detalhes bibliográficos
Autor principal: Costa, Ana Rita (author)
Outros Autores: Milho, Catarina (author), Azeredo, Joana (author), Pires, Diana Priscila Penso (author)
Formato: bookPart
Idioma:eng
Publicado em: 2018
Assuntos:
Texto completo:http://hdl.handle.net/1822/56504
País:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/56504
Descrição
Resumo:Recent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized bacteriophages with new functionalities. Bacteriophages have been engineered towards a wide range of applications including pathogen control and detection, targeted drug delivery, or even assembly of new materials. In this chapter, two strategies that have been successfully used to genetically engineer bacteriophage genomes are addressed: a yeast-based platform and bacteriophage recombineering of electroporated DNA.