Development and evaluation of a normal phase liquid chromatographic method for the determination of tocopherols and tocotrienols in walnuts
A high performance liquid chromatographic (HPLC) method for the determination of tocopherols and tocotrienols in walnut samples is described. The compounds were extracted with n-hexane, using a simple solid-liquid extraction procedure. Tocol was used as internal standard and BHT as anti-oxidant. The...
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| Other Authors: | , , |
| Format: | article |
| Language: | eng eng |
| Published: |
2008
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10198/632 |
| Country: | Portugal |
| Oai: | oai:bibliotecadigital.ipb.pt:10198/632 |
| Summary: | A high performance liquid chromatographic (HPLC) method for the determination of tocopherols and tocotrienols in walnut samples is described. The compounds were extracted with n-hexane, using a simple solid-liquid extraction procedure. Tocol was used as internal standard and BHT as anti-oxidant. The ehromatographic separation was achieved using an Inertsil 5 SI normal phase column operating with isocratic elution of n-hexane/1,4-dioxane (96.5:3.5, v/v), at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diodearray detector followed by a fluorescence detector. The detection limits were low, between 0.037 and 0.266 (jig/mL. The method was precise (% CV less than2.8%), accurate (% CV less than 5.6%), and, as a general rule, the recovery values were high (mean values ranging from 93.4% to 104%). |
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