Desorption of cellulases from cotton powder
Cotton fabrics were treated with three different Trichoderma reesei cellulase preparations (total crude – TC, endoglucanase enriched – EG-rich, cellobiohydrolase enriched – CBH-rich) using mechanical agitation to produce cotton powder. Desorption of cellulase enzymes from the cotton powder was then...
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| Outros Autores: | , |
| Formato: | article |
| Idioma: | eng |
| Publicado em: |
2001
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| Assuntos: | |
| Texto completo: | http://hdl.handle.net/1822/2607 |
| País: | Portugal |
| Oai: | oai:repositorium.sdum.uminho.pt:1822/2607 |
| Resumo: | Cotton fabrics were treated with three different Trichoderma reesei cellulase preparations (total crude – TC, endoglucanase enriched – EG-rich, cellobiohydrolase enriched – CBH-rich) using mechanical agitation to produce cotton powder. Desorption of cellulase enzymes from the cotton powder was then performed by washing with buffer. After 3 washings most of the protein was desorbed from the cotton powder and the amount of sugars released in the latter washings was negligible. TC and CBH-rich preparations produced a finer cellulose powder than EGs. The desorption process caused a decrease in degree of polymerisation (DP) specially for the cotton treated with EGs and a marked increase in polydispersity (Pd ) for all preparations. |
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