Novel aqueous two-phase systems based on tetrahydrofuran and potassium phosphate buffer for purification of lipase

Aqueous two-phase systems (ATPS) based on tetrahydrofuran (THF) + potassium phosphate buffer (pH 7) were used in this work for the purification of lipases. Binodal curve, tie lines and critical point were obtained for the new THF-salt ATPS at 25 degrees C and the binodal curve were successfully corr...

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Bibliographic Details
Main Author: Souza, Ranyere L. (author)
Other Authors: Lima, Rafaella A. (author), Coutinho, Joao A. P. (author), Soares, Cleide M. F. (author), Lima, Alvaro S. (author)
Format: article
Language:eng
Published: 1000
Subjects:
PORCINE PANCREATIC LIPASE
POLYETHYLENE-GLYCOL
PHASE-SEPARATION
IONIC LIQUIDS
INDUSTRIAL APPLICATIONS
PROTEIN SEPARATION
BIPHASIC SYSTEMS
TOLERANT LIPASE
PLUS WATER
EXTRACTION
Online Access:http://hdl.handle.net/10773/20770
Country:Portugal
Oai:oai:ria.ua.pt:10773/20770

Internet

http://hdl.handle.net/10773/20770

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