Effect of different toxic compounds on ATP content and acid phosphatase activity in axenic cultures of Tetrahymena pyriformis

The sensitivity of protozoa, and particularly ciliated protozoa, to environmental changes suggested a study on the physiological responses arising from exposure to toxic compounds. Tetrahymena pyriformis was used as a test organism in a set of miniaturized assays. The physiological response of this...

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Bibliographic Details
Main Author: Nicolau, Ana (author)
Other Authors: Mota, M. (author), Lima, Nelson (author)
Format: article
Language:eng
Published: 2004
Subjects:
Online Access:http://hdl.handle.net/1822/1426
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/1426
Description
Summary:The sensitivity of protozoa, and particularly ciliated protozoa, to environmental changes suggested a study on the physiological responses arising from exposure to toxic compounds. Tetrahymena pyriformis was used as a test organism in a set of miniaturized assays. The physiological response of this ciliate was assessed in terms of adenosine-5'-triphosphate content and acid phosphatase activity after exposure of the cultures of T. pyriformis to four toxicants: copper, zinc, Triton X-100, and cycloheximide. In the range of concentrations used, stimulation and inhibition of these two parameters were observed. The correlation between the two parameters is analyzed.