Optimization of a peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method for the detection of bacteria and disclosure of a formamide effect

Despite the fact that fluorescence in situ hybridization (FISH) is a well-established technique to identify microorganisms, there is a lack of understanding concerning the interaction of the different factors affecting the obtained fluorescence. In here, we used flow cytometry to study the influence...

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Detalhes bibliográficos
Autor principal: Rita S. Santos (author)
Outros Autores: Nuno Guimarães (author), Pedro Madureira (author), Nuno F. Azevedo (author)
Formato: article
Idioma:eng
Publicado em: 2014
Assuntos:
Texto completo:https://hdl.handle.net/10216/103095
País:Portugal
Oai:oai:repositorio-aberto.up.pt:10216/103095