Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells

Critical steps in resistance of mice against Toxoplasma gondii occur in the first 2 or 3 h after the pathogen has entered a cell that has been exposed to interferon γ (IFNγ). The newly formed parasitophorous vacuole is attacked by the IFNγ-inducible IRG proteins and disrupted, resulting in death of...

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Bibliographic Details
Main Author: Alvarez, Catalina (author)
Other Authors: Campos, Ana Claudia (author), Howard, Jonathan C (author), Loureiro, Joana (author), Müller, Urs Benedikt (author), Rodrigues, Ana Lina (author)
Format: bookPart
Language:eng
Published: 2020
Subjects:
Research Subject Categories::NATURAL SCIENCES
Toxoplasma gondii Parasitophorous vacuole Mouse Innate immunity IRG proteins Interferon-γ Diaphragm-derived cells Reactive necrosis Flow cytometry Immunofluorescence
Online Access:http://hdl.handle.net/10400.7/929
Country:Portugal
Oai:oai:arca.igc.gulbenkian.pt:10400.7/929
Description
Summary:Critical steps in resistance of mice against Toxoplasma gondii occur in the first 2 or 3 h after the pathogen has entered a cell that has been exposed to interferon γ (IFNγ). The newly formed parasitophorous vacuole is attacked by the IFNγ-inducible IRG proteins and disrupted, resulting in death of the parasite and necrotic death of the cell. Here we describe some techniques that we have used to describe and quantify these events in different combinations of the host and the parasite.

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