Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells

Critical steps in resistance of mice against Toxoplasma gondii occur in the first 2 or 3 h after the pathogen has entered a cell that has been exposed to interferon γ (IFNγ). The newly formed parasitophorous vacuole is attacked by the IFNγ-inducible IRG proteins and disrupted, resulting in death of...

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Detalhes bibliográficos
Autor principal: Alvarez, Catalina (author)
Outros Autores: Campos, Ana Claudia (author), Howard, Jonathan C (author), Loureiro, Joana (author), Müller, Urs Benedikt (author), Rodrigues, Ana Lina (author)
Formato: bookPart
Idioma:eng
Publicado em: 2020
Assuntos:
Research Subject Categories::NATURAL SCIENCES
Toxoplasma gondii Parasitophorous vacuole Mouse Innate immunity IRG proteins Interferon-γ Diaphragm-derived cells Reactive necrosis Flow cytometry Immunofluorescence
Texto completo:http://hdl.handle.net/10400.7/929
País:Portugal
Oai:oai:arca.igc.gulbenkian.pt:10400.7/929
Descrição
Resumo:Critical steps in resistance of mice against Toxoplasma gondii occur in the first 2 or 3 h after the pathogen has entered a cell that has been exposed to interferon γ (IFNγ). The newly formed parasitophorous vacuole is attacked by the IFNγ-inducible IRG proteins and disrupted, resulting in death of the parasite and necrotic death of the cell. Here we describe some techniques that we have used to describe and quantify these events in different combinations of the host and the parasite.

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