Development of a Fluorescence In Situ Hybridization protocol for simultaneous analysis of Gram-positive and Gram-negative bacteria

In the present study several FISH procedures, varying on the fixatives used (50,80 and 96% ethanol aqueous solutions), were investigated for analyzing both Gram-positive and Gram-negative bacteria cells harvested at different growth stages. The universal bacterial EUB338 Cy3-labeled probe were used...

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Bibliographic Details
Main Author: Pérez, Marina González (author)
Other Authors: Fernandes, Rafaela (author), Vieira, Ricardo (author), Pereira, António (author), Candeias, António (author), Caldeira, Ana T. (author)
Format: article
Language:eng
Published: 2016
Subjects:
FISH
Gram-positive
Gram-negative
bacteria
Online Access:http://hdl.handle.net/10174/17067
Country:Portugal
Oai:oai:dspace.uevora.pt:10174/17067

Internet

http://hdl.handle.net/10174/17067

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