Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform

In this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPs onto large unilamellar vesicle structures...

Full description

Bibliographic Details
Main Author: Barroso, M. Fátima (author)
Other Authors: Luna, M. Alejandra (author), Tabares, Juan S. Flores (author), Delerue-Matos, Cristina (author), Correa, N. Mariano  (author), Moyano, Fernando (author), Molina, Patricia G. (author)
Format: article
Language:eng
Published: 2017
Subjects:
Covalently decorated vesicles
Gold nanoparticles
Immunosensors design
Synthesis
Online Access:http://hdl.handle.net/10400.22/9254
Country:Portugal
Oai:oai:recipp.ipp.pt:10400.22/9254
Description
Summary:In this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPs onto large unilamellar vesicle structures is described. The vesicles were formed with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-undecanethiol (SH). After, the AuNPs photochemically synthesized in pure glycerol were mixed and anchored onto SH–DOPC vesicles. The data provided by voltammetry, spectrometry and microscopy techniques indicated that the AuNPs were successfully covalently anchored onto the vesicle bilayer and decorated vesicles exhibit a spherical shape with a size of 190 ± 10 nm. The developed procedure is easy, rapid and reproducible to start designing a possible immunosensor by using envi-ronmentally friendly procedures.

Similar Items