Direct kinetic assay of interactions between small peptides and immobilized antibodies using a surface plasmon resonance biosensor
A surface plasmon resonance (SPR) protocol is described for the direct kinetic analysis of small antigenic peptides interacting with immobilized monoclonal antibodies (mAb). High peptide concentrations (up to 2.5 muM) and medium mAb surface densities (about 1.5 ng/mm(2)) are needed to ensure measura...
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| Format: | article |
| Language: | eng |
| Published: |
2002
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| Online Access: | https://repositorio-aberto.up.pt/handle/10216/82023 |
| Country: | Portugal |
| Oai: | oai:repositorio-aberto.up.pt:10216/82023 |