Optimization of a microplate-based assay to assess esterase activity in the alga Pseudokirchneriella subcapitata

The present work describes the optimization of a short-term assay, based on the inhibition of the esterase activity of the alga Pseudokirchneriella subcapitata, in a microplate format. The optimization of the staining procedure showed that the incubation of the algal cells with 20 μmol L−1 fluoresce...

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Bibliographic Details
Main Author: Machado, Manuela D. (author)
Other Authors: Soares, Eduardo V. (author)
Format: article
Language:eng
Published: 2013
Subjects:
Online Access:http://hdl.handle.net/1822/24332
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/24332