Cloning and expression of an elongation factor-1α in sea bream ( Sparus aurata ) larvae and adult tissue

A clone encoding the polypeptide elongation factor EF-1a was isolated from a complementary DNA library prepared from sea bream (Spartus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1a in other animal species. EF-1a messenger RNA is...

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Bibliographic Details
Main Author: Nowell, M. A. (author)
Other Authors: Power, Deborah (author), Guerreiro, P. M. (author), Llewellyn, Lynda (author), Ramsurn, Vimi P. (author), Wigham, Trevor (author), Sweeney, Glen E. (author)
Format: article
Language:eng
Published: 2014
Subjects:
Online Access:http://hdl.handle.net/10400.1/5386
Country:Portugal
Oai:oai:sapientia.ualg.pt:10400.1/5386
Description
Summary:A clone encoding the polypeptide elongation factor EF-1a was isolated from a complementary DNA library prepared from sea bream (Spartus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1a in other animal species. EF-1a messenger RNA is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. This increase presumably reflects midblastula transition in this species. In adult sea bream, EF-1a appeared to have a relatively uniform distribution across all the tissues analyzed.