Decavanadate binding to a high affinity site near the myosin catalytic centre inhibits F-actin-stimulated myosin ATPase activity

Decameric vanadate (V10) inhibits the actin-stimulated myosin ATPase activity, noncompetitively with actin or with ATP upon interaction with a high-affinity binding site (Ki ) 0.27 ( 0.05 íM) in myosin subfragment-1 (S1). The binding of V10 to S1 can be monitored from titration with V10 of the fluor...

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Bibliographic Details
Main Author: Tiago, Teresa (author)
Other Authors: Aureliano, M. (author), Gutiérrez-Merino, Carlos (author)
Format: article
Language:eng
Published: 2012
Subjects:
Online Access:http://hdl.handle.net/10400.1/1358
Country:Portugal
Oai:oai:sapientia.ualg.pt:10400.1/1358