Direct single-step surface plasmon resonance analysis of interactions between small peptides and immobilized monoclonal antibodies

Surface plasmon resonance (SPR) methods have been optimized to permit direct kinetic analysis of the antigenic peptide analytes interacting with immobilized monoclonal antibodies (mAbs). High reproducibility and a significant correlation between SPR and previous ELISA data on the same set of antibod...

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Bibliographic Details
Main Author: Gomes, P (author)
Other Authors: Giralt, E (author), Andreu, D (author)
Format: article
Language:eng
Published: 2000
Subjects:
Medicina básica
Basic medicine
Online Access:https://hdl.handle.net/10216/82066
Country:Portugal
Oai:oai:repositorio-aberto.up.pt:10216/82066
Description
Summary:Surface plasmon resonance (SPR) methods have been optimized to permit direct kinetic analysis of the antigenic peptide analytes interacting with immobilized monoclonal antibodies (mAbs). High reproducibility and a significant correlation between SPR and previous ELISA data on the same set of antibodies and peptides were observed. The kinetic data obtained provide further insight into the structure of the main antigenic site of foot-and-mouth disease virus (FMDV).

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