Purification and mechanistic characterisation of two polygalacturonases from Sclerotium rolfsii

Sclerotium rolfsii (strain CBS 350.80) was found to produce extraordinary high amounts of polygalacturonases (PGs). Two of these extracellular enzymes were purified by a recently introduced preparative electrophoretic device (isoelectric focusing mode of free flow electrophoresis). PG 1 (39.5 kDa, p...

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Bibliographic Details
Main Author: Schnitzhofer, W (author)
Other Authors: Weber, H.-J. (author), Vršanská, M. (author), Biely, P. (author), Paulo, Artur Cavaco (author), Guebitz, G. M. (author)
Format: article
Language:eng
Published: 2007
Subjects:
Online Access:http://hdl.handle.net/1822/13560
Country:Portugal
Oai:oai:repositorium.sdum.uminho.pt:1822/13560