Visualizing Structures in Confocal Microscopy Datasets Through Clusterization: A Case Study on Bile Ducts

Aiming at a better result from previous works, we employed some heuristics found in the literature to determine the appropriate parameters for the clustering. We proposed our methodology by adding some steps to be performed before the clustering phase: one step for pre-processing the volumetric data...

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Bibliographic Details
Main Author: Beltran, Lizeth A.C. (author)
Other Authors: Cruz, Carolina U. (author), Santos, Jorge Luiz dos (author), Shivakumar, Pranavkumar (author), Bezerra, Jorge (author), Freitas, Carla M.D.S. (author)
Format: conferenceObject
Language:eng
Published: 2020
Subjects:
Online Access:http://hdl.handle.net/10400.6/9025
Country:Portugal
Oai:oai:ubibliorum.ubi.pt:10400.6/9025
Description
Summary:Aiming at a better result from previous works, we employed some heuristics found in the literature to determine the appropriate parameters for the clustering. We proposed our methodology by adding some steps to be performed before the clustering phase: one step for pre-processing the volumetric dataset and another to analyzing candidate features to guide the clustering. In this latter aspect, we provide an interesting contribution: we have explored the gradient magnitude as a feature that allowed to extract relevant information from the density-based spatial clustering. Besides the fact that DBSCAN allows easy detection of noise points, an interesting result for both datasets was that the first and largest cluster found as significant for the visualization represents the structure of interest. In the red channel, this cluster represents the most prominent vessels, while in the green channel, the peribiliary glands were made more evident.