Microsatellite markers for the giant kelp macrocystis pyrifera

We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic mic...

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Detalhes bibliográficos
Autor principal: Alberto, F. (author)
Outros Autores: Whitmer, A. (author), Coelho, N. C. (author), Zippay, M. (author), E, Varela-Álvarez (author), Raimondi, P. T. (author), Reed, D. C. (author), Serrão, Ester (author)
Formato: article
Idioma:eng
Publicado em: 2014
Assuntos:
Macrocystis pyrifera
Giant kelp
Microsatellites
Macroalga
Genetic diversity
Texto completo:http://hdl.handle.net/10400.1/4040
País:Portugal
Oai:oai:sapientia.ualg.pt:10400.1/4040
Descrição
Resumo:We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic microsatellites and are described. These loci identified a number of alleles ranging from three to forty (mean = 16.5, and gene diversity ranging from 0.469 to 0.930 (mean = 0.774). The isolation and characterization of these highly polymorphic markers will greatly benefit much needed studies on the molecular ecology of this important macroalga.

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