Characterization of a polyubiquitin gene in T. thermophila and of ubiquitin gene expression during sexual reproduction and under stress conditions

A 5-unit polyubiquitin gene, TTU3, was isolated from a T. thermophila genomic library and sequenced. This gene presents an extra triplet coding for Phe, a AGAGA motif and a putative HSE element in its 5'-non-coding region. The ubiquitin gene expression in this ciliate was investigated by Northe...

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Detalhes bibliográficos
Autor principal: Guerreiro, Paulo (author)
Outros Autores: Rodrigues-Pousada, Claudina (author)
Formato: article
Idioma:eng
Publicado em: 2014
Assuntos:
Ubiquitin mRNAs
Genomic organization
Tetrahymena thermophila
Ciliate
Hyperthermic stress
Amino acid sequence
Base sequence
Cloning, Molecular
Gene dosage
Gene expression regulation
Molecular sequence data
RNA, Messenger
Sequence analysis
Texto completo:http://hdl.handle.net/10400.21/3089
País:Portugal
Oai:oai:repositorio.ipl.pt:10400.21/3089
Descrição
Resumo:A 5-unit polyubiquitin gene, TTU3, was isolated from a T. thermophila genomic library and sequenced. This gene presents an extra triplet coding for Phe, a AGAGA motif and a putative HSE element in its 5'-non-coding region. The ubiquitin gene expression in this ciliate was investigated by Northern blot hybridization in conjugating cells or cells under stress conditions. Exponentially growing cells express two ubiquitin mRNAs of 0.75 and 1.8 kb and a new species of 1.4 kb is induced under hyperthermic stress. During sexual reproduction of the cells (conjugation) the 1.8-kb mRNA is still transcribed whereas the steady-state population of the 0.75 mRNA transcripts is strongly diminished. Southern blot analysis suggests that ubiquitin in T. thermophila constitutes a large family of about ten members.

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