Cell-free layer (CFL) analysis in a glass capillary: comparison between a manual and automatic method
In this study, in vitro blood flowing through a 100 m glass capillary was studied. The images were captured using a confocal system and post-processed using Image J and MatLab. The aim of the present work, was to measure the trajectories of the cell-free layer (CFL) by using two different methods, i...
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| Other Authors: | , , |
| Format: | conferenceObject |
| Language: | eng |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10198/11216 |
| Country: | Portugal |
| Oai: | oai:bibliotecadigital.ipb.pt:10198/11216 |
| Summary: | In this study, in vitro blood flowing through a 100 m glass capillary was studied. The images were captured using a confocal system and post-processed using Image J and MatLab. The aim of the present work, was to measure the trajectories of the cell-free layer (CFL) by using two different methods, i. e., a manual method (MM) and an automatic method (AM). For theMM we have used amanual tracking plugin (MTrackJ) from Image J to track labeled red blood cells (RBCs) flowing around the boundary of the RBCs core. For the AM we have used a MatLab scripts to measure automatically the CFL trajectories. The preliminary numerical results suggest that the CFL trajectories follow a polynomial function for both methods. |
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