A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers

We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the numbe...

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Bibliographic Details
Main Author: Deprá,Maríndia (author)
Other Authors: Sepel,Lenira Maria Nunes (author), Loreto,Élgion Lucio da Silva (author)
Format: article
Language:eng
Published: 2004
Subjects:
transgenic Drosophila
piggyBac
GFP
transposable elements
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012
Country:Brazil
Oai:oai:scielo:S1415-47572004000100012

Internet

http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012

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