UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation

ABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric method exploiting the Dushman reaction. Both methods were validated based on ICH guidelines and were successfully applied to quantitate the drug in marketed formulation...

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Bibliographic Details
Main Author: SIDDIQUI,Masoom Raza (author)
Other Authors: HAKAMI,Afnan Ali Hussain (author), WABAIDUR,Saikh Mohammad (author), ALOTHMAN,Zeid Abdullah (author), KHAN,Moonis Ali (author), HUSAIN,Fohad Mabood (author)
Format: article
Language:eng
Published: 2021
Subjects:
Ceftazidime
UPLC-MS/MS
method development
spectrophotometry
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100225
Country:Brazil
Oai:oai:scielo:S0101-20612021000100225
Description
Summary:ABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric method exploiting the Dushman reaction. Both methods were validated based on ICH guidelines and were successfully applied to quantitate the drug in marketed formulation. UPLC-MS/MS method responded linearly in the range of 1.6 µgmL-1-6.4 µgmL-1 while spectrophotometric procedure followed the Beer’s law in the concentration range of 30-100 µgmL-1. UPLC-MS/MS has been found to have a limit of quantitation of 0.97 µgmL-1 while spectrophotometric method was found to quantitate 14.00 µgmL-1 correctly with high precision. Both the methods showed an excellent recovery of 99.36%-100.91% (% RSD 1.05-1.73) and 98.64%-99.73% (%RSD 0.67-1.68) for UPLC-MS/MS and spectrophotometric, respectively.

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