Role of adiponectin in diabetes myocardial ischemia-reperfusion injury and ischemic postconditioning

Abstract Purpose Patients with diabetes are vulnerable to myocardial I/R (ischaemia/reperfusion) injury, but are not responsive to IPO (ischaemic post-conditioning). We hypothesized that decreased cardiac Adiponectin (APN) is responsible for the loss of diabetic heart sensitivity to IPO cardioprotec...

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Detalhes bibliográficos
Autor principal: Cao,Chen (author)
Outros Autores: Liu,Hui-min (author), Li,Wei (author), Wu,Yang (author), Leng,Yan (author), Xue,Rui (author), Chen,Rong (author), Tang,Ling-hua (author), Sun,Qian (author), Xia,Zhongyuan (author), Tang,Qi-zhu (author), Shen,Di-fei (author), Meng,Qing-tao (author)
Formato: article
Idioma:eng
Publicado em: 2020
Assuntos:
Adiponectin
Diabetes
Reperfusion Injury
Ischemic Postconditioning
Texto completo:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020000100204
País:Brasil
Oai:oai:scielo:S0102-86502020000100204
Descrição
Resumo:Abstract Purpose Patients with diabetes are vulnerable to myocardial I/R (ischaemia/reperfusion) injury, but are not responsive to IPO (ischaemic post-conditioning). We hypothesized that decreased cardiac Adiponectin (APN) is responsible for the loss of diabetic heart sensitivity to IPO cardioprotecton. Methods Diabetic rats were subjected to I/R injury (30 min of LAD occlusion followed by 120 min of reperfusion). Myocardial infarct area was determined by TTC staining. Cardiac function was monitored by a microcatheter. ANP, 15-F2t-isoprostane, nitrotyrosine and MDA were measured by assay kits. Levels of p-Akt, total-Akt and GAPDH were determined by Western Blot. Results Diabetic rats subjected to myocardial IR exhibited severe myocardial infarction and oxidative stress injury, lower APN in the plasma and cardiac p-Akt expression ( P <0.05). IPO significantly attenuated myocardial injury and up-regulated plasma APN content and cardiac p-Akt expression in non-diabetic rats but not in diabetic rats. Linear correlation analysis showed that the expression of adiponectin was positively correlated with p-Akt and negatively correlated with myocardial infarction area ( P <0.01). Conclusion Protective effect of IPO was tightly correlated with the expression of adiponectin, exacerbation of I/R injury and ineffectiveness of IPO was partially due to the decline of adiponectin and inactivation of Akt in diabetes mellitus.

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