Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography

This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded...

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Detalhes bibliográficos
Autor principal: Moreira,Keila Aparecida (author)
Outros Autores: Diogo,Margarida (author), Prazeres,Duarte Miguel (author), Lima Filho,José Luiz de (author), Porto,Ana Lúcia Figueiredo (author)
Formato: article
Idioma:eng
Publicado em: 2005
Assuntos:
Texto completo:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400014
País:Brasil
Oai:oai:scielo:S1516-89132005000400014